Education
B.S., Chemistry, Osmania University, India, 1983
M.S., Zoology, Kakatiya University, India, 1986
Ph.D., Zoology (Insect Toxicology), Osmania University, India, 1991
Research Focus
Our research focuses on developing, directing and conducting state-of-the-art research and development in nanobiotechnology, nanotoxicity, and synthesis of nanotechnology targeted antimicrobial agents. The main focus is on the engineered nanomaterials (NM), in the range of 1-100 nm in size, possessing novel physical and chemical properties that have been used to create unique devices. Unique quantum properties of nanomaterials strongly influence their physico-chemical properties, conferring electrical, optical and magnetic properties not present in corresponding bulk materials at a larger scale.
- To develop innovative toxicity testing methodologies to accurately predict the human health risks of new Air Force Systems, materials and technologies. Assessment of potential toxicities arising from the physicochemical properties uniquely associated with nanoscale structures. These efforts characterize effects and interactions of nanomaterials on natural systems to support the development of biosensors, taking into account the environmental, safety and health effects (ES&H) criteria for military operations.
- To develop fundamental understanding of the interaction of
nanoparticles with living cells that represent simple bacteria to mammalian
cells with reference to their uptake, translocation, distribution and potential
toxicity. This research will facilitate a better understanding of nano-bio
interaction mechanisms, provide in-depth analyses of corresponding effects on
biological systems and enable the theoretical development of predictive
bioresponse models. The understanding of how engineered nanoparticles of
different geometries interact with cells. Such knowledge will not only help to
improve nanomaterial safety strategies for the protection of both human and
environmental health, but will also help to apply advanced nanobiotechnology to
the development of future unique biosensors.
- To further understand how these particles interact with
cellular proteins and genes based on their physico-chemical properties
(particle number, particle size distribution, particle shape, surface area of
the primary particle and agglomerated material, intracellular
agglomeration/de-agglomeration status, charge and chemistry). To better
understand molecular events involved in nanoparticle– membrane receptor
binding, endocytosis and subsequent signaling activation. The studies also
emphasize the unique structure and the outstanding electronic radio frequency
properties of nanomaterials coupled with biomolecules (i.e., DNA or proteins)
and their potential application as gene or protein and receptor regulators.
Techniques
- Routine analysis of cellular in vitro tests in various cells
types (rat liver cells, alveolar macrophages, PC-12 cells, and germ-line cells)
as representatives of potential target organs (e.g., ingestion, inhalation,
intramuscular, reproductive etc.,) using broad spectrum of toxicity end points.
- Development of innovative improved cell-based assays by
using high-content image analysis for toxicity evaluation of nanomaterials
- Conduct studies to evaluate the mechanism of toxicity of new
chemical entities that involves binding and interaction with cellular proteins
and receptors, and the biochemical and physiological consequences of these
actions. The goal is to develop more sensitive in vitro cell models.
- Characterization of nanomaterials in biological system with
reference to nanotoxicity, using dynamic light scattering techniques
- Uptake and translocation of nanomaterials by using Cytoviva
imaging, confocal, SEM, TEM and AFM.
- Development of co-cultures to more accurately predict the in
vivo biological responses following exposure to nanomaterials (lung, skin, and
germ-line co-culture).
- Assessment of in-depth biological responses to newly synthesized
chemicals using quantitative PCR, ELISAs, western blotting, and fluorescent-based
technology to target changes in gene expression and cell signaling pathways.
Recent Publications
Schrand
AM, Huang H, Carlson C, Schlager JJ, Osawa O, Hussain SM and Dai L (2006) Are diamond nanoparticles
cytotoxic? J Phys Chem B 111:2-7.
Hussain SM, Javorina A, Schrand AM, Duhart H, Ali SF and
Schlager JJ (2006) The interaction of
manganese nanoparticles with PC-12 cells induces dopamine depletion. Toxicol
Sci 92:456-463.
Wagner AJ, Bleckmann CA, Murdock RC, Schrand AM,
Schlager JJ and Hussain SM (2007)
Cellular interaction of aluminum and aluminum oxide (Al2O3) nanoparticles. J
Phys Chem B 111:7353-7359.
Skebo JE, Grabinski CM, Schrand AM, Schlager JJ and Hussain
SM (2007) Assessment of
nanoparticle agglomeration, uptake, and interaction using a high illuminating
system. Int J Toxicol 26:151-158.
Murdock RC, Braydich-Stolle L, Schrand AM, Schlager
JJ and Hussain SM (2007)
Characterization of nanomaterial dispersion in solution prior to in vitro exposure using dynamic light scattering technique. Toxicol
Sci 101:239-253.
Grabinski C, Hussain S, Lafdi K, Braydich-Stolle L and Schlager JJ (2007)
Effect of Dimension on Biocompatibility of Carbon Nanomaterials. Carbon 45:2828-2835.
Schrand AM, Dai L, Schlager JJ, Hussain SM and Osawa E (2007) Differential biocompatibility of
carbon nanotubes and nanodiamonds. Diamond and Related Materials 16:2118.
Schrand AM, Szcublewski K, Schlager JJ, Dai L and Hussain
SM (2007) Interaction and
biocompatibility of multi-walled carbon nanotubes in PC-12 Cells. Intl J
Neuroprotect Neurogen 3:115-121.
Schrand AM, Braydich-Stolle LK, Schlager JJ, Dai L
and Hussain SM (2008) Can silver
nanoparticles be useful as potential biological labels? Nanotechnol 19:104-117.
Rogers JE, Parkinson CV, Choi YW, Speshock JKL and Hussain
SM (2008) A preliminary assessment
of silver nanoparticle inhibition of monkeypox virus plaque formation. Nanoscale
Res Lett 3:129-133.
Carlson C, Hussain SM, Schrand A, Braydich-Stolle L, Hess K, Jones R and
Schlager J (2008) Unique cellular interaction of silver nanoparticles: size
dependent generation of reactive oxygen species. J Phys Chem B 112, 13608–13619
Ahamed M, Karns M, Goodson M, Rowe J, Hussain SM, Schlager JJ and Hong Y (2008) DNA damage response
to different surface chemistry of silver nanoparticles in mammalian cells. Toxicol
and Appl Pharm 233, 404–410
Braydich-Stolle LK. Schaeubline NM, Murdock RC, Jiang
J, Biswas P, Schlager JJ, Hussain SM (2008). Cyrstal structure mediates mode of cell death in titanium
dioxide nanotoxicity. J Nanopart Res (in press)
Shelley ML; Wagner AJ, Hussain SM, Bleckmann C (2008) Modeling the in vivo case with in
vitro nanotoxicity data. Int J Toxicol 27(5):359-67.
Chunhui Liu, Haiying He, Ravindra Pandey, Saber
Hussain, Shashi Karna (2008)
Interaction of Metallic Nanoparticles with a Biologically Active Molecule,
Dopamine. J Phys Chem B. 112, 15256–15259
Yu KO, Grabinski CM, Schrand AM, Murdock RC, Wang W,
Gu B, Schlager JJ and Hussain SM (2009) Toxicity of amorphous silica nanoparticles in mouse keratinocytes. J
Nanopart Res 11, 15-24, 2009
M. F. Rahman, J. Wang, T. A. Patterson, B. Robinson,
G. D. Newport, R. C. Murdock, J. J. Schlager, S. M. Hussain, and S. F. Ali (2008) Expression of genes related to
oxidative stress in the mouse brain after exposure to silver-25 nanoparticles.
Tox Letters (In press)
Hussain SM, Braydich-Stolle LK, Schrand AM, Murdock RC, Yu KO, Mattie DM, Schlager
JJ, Mauricio Terrones (2009) Toxicity Evaluation for Safe Use of Nanomaterials:
Recent Achievements and Technical Challenges. Advanced Materials (In Press)
SM Hussain and JJ Schlager (2009) Safety Evaluation of Silver Nanoparticles:
Inhalation Model for Chronic Exposure. Toxicol Sci (In Press: available online)
If you are US
citizen or permanent resident and interested to pursue research in
nanobiotechnology and nanotoxicity, don’t hesitate to forward a copy of your
resume to saber.hussain@wpafb.af.mil |
For more information, contact:
Saber M. Hussain, Ph.D.
711th Human Performance Wing
Air Force Research Laboratory/HEPB
Wright Patterson Air Force Base
Dayton, OH 45433-5707
(937) 904-9517
E-Mail: saber.hussain@wpafb.af.mil
|
