Education
B.S., Chemistry, Osmania University, India, 1983
M.S., Zoology, Kakatiya University, India, 1986
Ph.D., Zoology (Insect Toxicology), Osmania University, India, 1991
Research Focus:
- In-vitro toxicology area for measuring novel toxicity assays
- Conducting mechanistic studies in cultured cells.
“The goal is to develop innovative toxicity testing methodologies
to accurately predict the human health risks of new Air Force Systems,
materials and technologies.”
 Routine analysis of cellular in vitro tests in various cells types (Rat
liver cells, Alveolar macrophages, PC12 cells, and germ line cells) as
representative of potential target organs (e.g., ingestion, inhalation,
intramuscular, reproductive etc.,) using a spectrum of toxicity end points.
Development of innovative improved cell based assays by using image analysis
for toxicity evaluation of newly synthesized chemicals. Conduct studies
to evaluate the mechanism of toxicity of new chemical entities that involves
binding and interaction with cellular proteins and receptors, and the biochemical
and physiological consequences of these actions. The goal is to develop
sensitive in vitro cell models.
Current research project: Assess toxicity levels of nanomaterials
in cultured mammalian cells
 Nanomaterials, which range in size from 1 - 100 nanometers, have been
used to create unique devices at the nanoscale level possessing novel physical
and chemical functional properties. Although nanomaterials are currently
being widely used in modern technology, there is a serious lack of information
concerning the human health and environmental implications of manufactured
nanomaterials. In view of their possible effect on human health
my main focus is to define and/or classify nanoparticles based on nature
of toxicity in in-vitro models. Identify the general mechanism
of toxicity whether oxidative stress, apoptosis or receptor mediated toxicity
involved by using innovative cell biology techniques
Selected Publications:
Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager
JJ. (2005) In Vitro Toxicity of Nanoparticles in BRL 3A Rat Liver
Cells. Toxicology
In Vitro 19; 975-983.
Braydich-Stolle L, Hussain S, Schlager J, Hofmann M-C. (2005) In Vitro Cytotoxicity of Nanoparticles In Mammalian Germ-Line Stem Cells. Tox. Sci. 88(2):
412-419.
Tan Y , Shi L, Hussain S, Xu J,
Tong W, Frazier JM, and Wang C (2006). Integrating
time-course microarray gene expression profiles with cytotoxicity. Bioinformatics 2006; 22: 77-87.
Hussain SM, Frazier JM. (2003) Involvement of apoptosis in hydrazine induced toxicity in rat primary hepatocytes. Toxicology in Vitro17, 343-355.
Hussain SM, Ali SF. (2002) Zinc potentiates MPTP induced dopamine depletion in caudate nucleus of mice brain. Neurosci. Lett. 335, 25-28.
Hussain SM, Frazier JM. (2002) Cellular toxicity of hydrazine in primary hepatocytes. Tox. Sci. 69, 424-432.
Witzmann FA, Clack JW, Geiss K, Hussain S, Juhl MJ, Rice CM, Wang C. (2002) Proteomic evaluation of cell preparation methods in primary hepatocyte cell culture. Electrophoresis 23, 22232232
Trohalaki S, Pachter R, Hussain S, Frazier J. (2002) Quantitative structure activity relationships (QSARS) for high-energy chemicals. Tox. Sci. 68, 498-507.
Hussain SM, Frazier JM. (2001) In vitro assessment of high energy chemicals in rat hepatocytes. Sci. Total Environ. 274, 151-160.
Haraguchi Y, Sakurai H, Hussain S, Anner BM, Hoshino H. (1999) Inhibition of HIV-1 infection by zinc group metal compounds. Antiviral Res. 43(2): 123-33.
Hussain S, Atkinson A, Khan A, Thompson S. (1999) Accumulation of mercury and its effect on antioxidant enzymes in brain, liver, kidneys of mice. J. Environ. Sci. Hlth. B, 645-660.
Hussain S, Hass B, Slikker W Jr., Ali SF. (1999) Reduced levels of catalase activity potentiate MPP+ toxicity: Comparison between MN9D cells and CHO cells. Toxicology Letters 104, 49-56.
Hussain S, Ali SF. (1999) Manganese scavenges superoxide and hydroxyl radicals: An in vitro study in rats. Neurosci. Lett. 261, 21-24.
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