Department of Biochemistry & Molecular Biology

Michael Leffak, Ph.D., Interim Chair

Nick Reo, Ph.D.

Nicholas V. Reo, Ph.D.

Professor
Director, Magnetic Resonance Laboratory

Affiliated Appointments:
Department of Physics
Department of Biomedical, Industrial & Human Factors Engineering
Main Office & Lab:
239 MR Laboratory
Cox Institute
3525 Southern Blvd,
Dayton, OH 45429

(937) 395-8038
nicholas.reo@wright.edu

Education

Postdoctoral Research Associate, March 1983–October 1985
Department of Chemistry, Washington University, St. Louis, Missouri
Mentor: Dr. Joseph J. H. Ackerman

Ph.D., Physical Chemistry, 1983
University of Massachusetts, Amherst, Massachusetts
Mentor: Dr. Thomas R. Stengle

M.S., Chemistry, 1981
University of Massachusetts, Amherst, Massachusetts

B.A., Chemistry, 1978
Rutgers University, Newark, New Jersey

Research Interests

NMR-based Metabolomics and Tissue Metabolism

In general, I am interested in studies of tissue metabolism using NMR spectroscopy. We are developing NMR-based metabolomics techniques in support of predictive toxicology, and as a means to monitor metabolic changes in response to various stimuli.

NMR Facility

The WSU Magnetic Resonance Laboratory houses two research instruments: (1) a 8.5 Tesla Wide-Bore (89 mm clear bore) 360 MHz NMR System equipped with a Tecmag Discovery Console; and (2) a Varian INOVA 600 NMR Spectrometer.

The 360 NMR system has various Bruker commercial probes for proton, fluorine, and broadband capabilities. This system is also used for NMR studies in vivo and is equipped with several home-built probes that can accommodate mice or rats.

The Varian INOVA 600 is equipped with: (1.) a triple resonance inverse probe (1H/13C/X), (2.) a broadband observe probe with a 13C/1H decouple channel (X/13C/1H), (3.) an Inverse Nanoprobe for 1H MAS spectroscopy in small volumes (40 ul), (4.) a variable temperature unit (FTS Systems, XR401 Air-Jet Crystal Cooler, -40 °C to +100 °C), and (5.) a Zymark XP Robotics Sample Changer (50 sample capacity.)

Current Projects

  1. Development of NMR-based metabolomics in toxicity and disease.
  2. NMR-based metabolomics analyses of biofluids (urine, plasma, sera) in relation to organ toxicity (collaboration with the Air Force Research Laboratory).
  3. Studies of brain phospholipid metabolism and effects of oxidative stress. 
  4. Studies of estrogenic endocrine disruptors (EEDs) using gene arrays, NMR metabolite analyses, and clinical chemistry. This collaborative project involves investigators at WSU, Michigan State U, the AFRL, and BAE Systems, Inc. We are employing a "systems biology" approach to examine multiple changes in tissues (primarily liver) in response to EEDs.

Representative Publications

P. Anderson, N.V. Reo, and M.L. Raymer. "A New Gaussian Method for Analysis of NMR Spectra for Metabolomics Studies." Metabolomics (submitted).

M. Westrick, N.J. DelRaso, M.L. Raymer, A.E. Neuforth, D.A. Mahle, and N.V. Reo. "Dose and Time Response Metabolomic Analyses of a-Napthylisothiocyanate Toxcity in the Rat." Chem. Res. Toxicol. (submitted).

B. Hoffman-Kuczynski and N. V. Reo. "Evidence that Plasmalogen Protects Against Oxidative Stress in the Rat Brain in Vivo." Neurochem. Res. 31, 639-656 (2006).

B. Hoffman-Kuczynski and N. V. Reo. "Studies of Myo-Inositol and Plasmalogen Metabolism in Rat Brain." Neurochem. Res. 29, 843-855 (2004).

B. Hoffman-Kuczynski and N. V. Reo. "Administration of Myo-Inositol Plus Ethanolamine Elevates Phosphatidylethanolamine Plasmalogen in the Rat Cerebellum." Neurochem. Res. 30 (1), 47-60 (2005).

N. V. Reo, M. Adinehzadeh, and B. D. Foy. "Kinetic Analyses of Liver Phosphatdiylcholine and Phosphatidylethanolamine Biosynthesis Using 13C NMR Spectroscopy." Biochim. Biophys. Acta. (Mol. Cell Biol. Lipids), 1580, 171-188 (2002).

N. V. Reo. "NMR-Based Metabolomics." Proceedings from "Issues and Applications in Toxicology and Risk Assessment." Drug Chem. Toxicol. 25 (4), 375-382 (2002).

N. V. Reo and M. Adinehzadeh. "NMR Spectroscopic Analyses of Liver Phosphatidylcholine and Phosphatidylethanolamine Biosynthesis in Rats Exposed to Peroxisome Proliferators - a Class of Nongenotoxic Hepatocarcinogens." Toxicol. Appl. Pharmacol. 164, 113-126 (2000).