Ph.D.: University of Illinois at Urbana-Champaign
Postdoctoral: The Ohio State University
Research Interests:
Our group is using a molecular genetic approach to study the sulfur
regulatory system of the filamentous fungus Neurospora crassa. The
sulfur regulatory system is made up of a set of trans-acting negative
and positive regulatory genes that control a number of structural genes
involved in sulfur acquisition and processing. We have concentrated on
cloning the components of the control system and studying the genes at
the molecular level. The cloned genes currently under study include the
sulfur controller-2 negative regulatory gene which encodes a protein
with F-box and WD40 domains, the cys-3 positive regulatory gene which
encodes a DNA binding protein with a leucine zipper element, and the
arylsulfatase structural gene. The studies being conducted are aimed
at achieving an understanding of the novel regulatory interactions and
organization of this eukaryotic multigene network.
Selected References:
Paietta, John V. (2008) DNA-binding
specificity of the CYS3
transcription factor of Neurospora crassa defined by binding-site
selection. Fungal Genetics and Biology 45: 1166-1171. [Free
full-text article.]
Paietta, JV. (2004) Regulation of sulfur metabolism in mycelial
fungi. In The Mycota: Biochemistry and Molecular Biology, Vol.
3A. Springer-Verlag, Berlin, p. 369-383.
Borkovich KA, Alex LA, Yarden O, Freitag M, Turner GE, Read ND, Seiler
S, Bell-Pedersen D, Paietta JV, Plesofsky N, Plamann
M, Goodrich-Tanrikulu M, Schulte U, Mannhaupt G, Nargang FE, Radford
A, Selitrennikoff C, Galagan JE, Dunlap JC, Loros JJ, Catcheside D, Inoue
H, Aramayo R, Polymenis M, Selker EU, Sachs MS, Marzluf GA, Paulsen I,
Davis R, Ebbole DJ, Zelter A, Kalkman ER, O'Rourke R, Bowring F, Yeadon
J, Ishii C, Suzuki K, Sakai W, and Pratt R. (2004) Lessons from the Genome
Sequence of Neurospora crassa: Tracing the Path from Genomic
Blueprint to Multicellular Organism. Microbiol. Mol. Biol. Rev. 68:1-108.
[Free full-text
article.]
Sizemore ST and Paietta JV. (2002) Cloning and characterization
of scon-3+, a new member of the Neurospora crassa sulfur
regulatory system. Eukaryotic Cell 1: 875-883. [Free
full-text article.]
Kumar A and Paietta JV. (1998) An additional role for the F-box
motif: Gene regulation within the Neurospora crassa sulfur control
network. Proc. Natl. Acad. Sci. USA 95, 2417-2422. [Free
full-text article.]
Kumar A and Paietta JV. (1995) The sulfur controller-2 negative
regulatory gene of Neurospora crassa encodes a protein with beta-transducin
repeats. Proc. Natl. Acad. Sci. USA 92, 3343-3347. [Free
full-text article.]
Paietta JV. (1995) Analysis of CYS3 regulator function in Neurospora
crassa by modification of leucine zipper dimerization specificity. Nucleic
Acids Res. 23(6):1044-9. [Free
full-text article.]
Paietta JV. (1992) Production of the CYS3 regulator, a bZIP DNA-binding
protein, is sufficient to induce sulfur gene expression in Neurospora
crassa. Mol Cell Biol. 12(4):1568-77. [Free
full-text article.]
Paietta JV. (1990) Molecular cloning and analysis of the scon-2
negative regulatory gene of Neurospora crassa. Mol Cell Biol. 10(10):5207-14.
[Free
full-text article.]
Paietta JV. (1989) Molecular cloning and regulatory analysis
of the arylsulfatase structural gene of Neurospora crassa. Mol
Cell Biol. 9(9):3630-7. [Free
full-text article.]
Fu, Y-H, Paietta, JV, Mannix, DG, Marzluf, GA (1988)
cys-3, the positive-acting sulfur regulatory gene of Neurospora crassa,
encodes a protein with a putative leucine zipper DNA-binding element. Mol.
Cell. Biol. 9: 1120-1127. [Free
full-text article.]
Paietta JV, Akins RA, Lambowitz AM, Marzluf GA. (1987) Molecular
cloning and characterization of the cys-3 regulatory gene of Neurospora
crassa. Mol Cell Biol. 7(7):2506-11. [Free
full-text article.]
Paietta JV, Marzluf GA. (1985) Gene disruption by transformation
in Neurospora crassa. Mol Cell Biol. 5(7):1554-9. [Free
full-text article.]
Paietta JV, Marzluf GA. (1985) Plasmid
recovery from transformants and the isolation of chromosomal DNA segments
improving plasmid replication in Neurospora crassa. Curr Genet. 9(5):383-8.
[Free
full-text article.]
Paietta, J.V. and G.A. Marzluf (1985) Development of shuttle
vectors and gene manipulation techniques for Neurospora crassa.
In Molecular Genetics of Filamentous Fungi. Alan R. Liss, Inc.:
New York, p. 1-13.
Dhawale, S.S., Paietta, J.V. and G.A. Marzluf (1984) A new, rapid
and efficient transformation procedure for Neurospora. Current
Genetics 8: 77-79. [Free
full-text article.]
Paietta, J.V. and G.A. Marzluf (1984) Development of a colony
hybridization technique for Neurospora. Fungal Genet. Newsl.
31: 40. [Free full-text
article.]
Paietta, J.V. and G.A. Marzluf (1984) Transformation of lithium
acetate-treated Neurospora with minipreps of plasmid DNA. Fungal
Genet. Newsl. 31:40-41. [Free
full-text article.]
Paietta JV, Sargent ML. (1983) Isolation and characterization
of light-insensitive mutants of Neurospora crassa. Genetics. 104(1):11-21.
[Free full-text
article.]
Paietta, J.V. and M.L. Sargent (1983) Modification of blue light
responses by riboflavin analogs in Neurospora crassa. Plant
Physiol. 72: 764-766. [Free
full-text article.]
Paietta, J.V. and M.L. Sargent (1982) Blue light responses in
nitrate reductase mutants of Neurospora crassa. Photochem. Photobiol.
35: 853-855.
Paietta JV. (1982) Photooxidation and the evolution of circadian
rhythmicity. J Theor Biol. 97(1):77-82. [Abstract.]
Paietta JV, Sargent ML. (1981) Photoreception in Neurospora
crassa: correlation of reduced light sensitivity with flavin deficiency. Proc
Natl Acad Sci U S A. 78(9):5573-7. [Free
full-text article.]
Pao CC, Paietta JV, Gallant JA. (1977) Synthesis of guanosine
tetraphosphate (magic spot I) in Saccharomyces cerevisiae. Biochem
Biophys Res Commun. 74(1):314-22. [Abstract.]
Paietta JV, McDonald TL, Doermann AH. (1976) Method for the isolation
of bacteriphage T4 mutants that produce particles with giant capsids. J
Virol. 18(2):785-7. [Free
full-text article.]
Clayton DL, Paietta JV. (1972) Selection for circadian eclosion
time in Drosophila melanogaster. Science. 178(64):994-5.
[Abstract.]
Nucleotide Sequences:
1. AY135642
Neurospora crassa rip12b-1 mutant sulfur controller-3
(scon-3) mRNA, complete cds
gi|27501467|gb|AY135642.1|[27501467]
2. AF402682
Neurospora crassa negative regulator sulfur controller-3
(scon-3) gene, complete cds
gi|22086559|gb|AF402682.1|[22086559]
3. AY052777
Neurospora crassa sulfite reductase (cys-4) gene,
complete cds
gi|19068027|gb|AY052777.1|[19068027]
4. AF404820
Neurospora crassa methionine synthase (met-8) gene,
complete cds
gi|17385933|gb|AF404820.1|AF404820[17385933]
5. AF401238
Neurospora crassa cystathionine gamma-lyase (cys-16)
gene, complete cds
gi|15278112|gb|AF401238.1|AF401238[15278112]
6. AF401237
Neurospora crassa cystathionine beta-lyase (met-2)
gene, complete cds
gi|15278107|gb|AF401237.1|AF401237[15278107]
7. AY050220
Neurospora crassa homoserine o-acetyltransferase (met-5)
gene, complete cds
gi|15982696|gb|AY050220.1|[15982696]
8. U89492
Neurospora crassa arylsulfatase (ars-1) gene, complete
cds
gi|2873362|gb|U89492.1|NCU89492[2873362]
9. X62506
N.crassa DNA for autonomously replicating sequence
gi|2980|emb|X62506.1|NCARSDNA[2980]
10. U17251
Neurospora crassa negative regulator sulfur controller-2
(scon-2) gene, complete cds
gi|806757|gb|U17251.1|NCU17251[806757]
11. M26008
Neurospora crassa cys-3 gene, complete cds
gi|168807|gb|M26008.1|NEUCYS3[168807] |
