Department of Biochemistry & Molecular Biology

Michael Leffak, Ph.D., Interim Chair

Dr. John Paietta

John V. Paietta, Ph.D.

Associate Professor

Office: 054 Diggs Lab
Lab: 025 Diggs Lab
(937) 775-3026
E-mail: Dr. Paietta


Ph.D.: University of Illinois at Urbana-Champaign
Postdoctoral: The Ohio State University

Research Interests:

Our group is using a molecular genetic approach to study the sulfur regulatory system of the filamentous fungus Neurospora crassa. The sulfur regulatory system is made up of a set of trans-acting negative and positive regulatory genes that control a number of structural genes involved in sulfur acquisition and processing. We have concentrated on cloning the components of the control system and studying the genes at the molecular level. The cloned genes currently under study include the sulfur controller-2 negative regulatory gene which encodes a protein with F-box and WD40 domains, the cys-3 positive regulatory gene which encodes a DNA binding protein with a leucine zipper element, and the arylsulfatase structural gene. The studies being conducted are aimed at achieving an understanding of the novel regulatory interactions and organization of this eukaryotic multigene network.

Selected References:

Paietta, John V. (2008) DNA-binding specificity of the CYS3 transcription factor of Neurospora crassa defined by binding-site selection. Fungal Genetics and Biology 45: 1166-1171. [Free full-text article.]

Paietta, JV. (2004) Regulation of sulfur metabolism in mycelial fungi. In The Mycota: Biochemistry and Molecular Biology, Vol. 3A. Springer-Verlag, Berlin, p. 369-383.

Borkovich KA, Alex LA, Yarden O, Freitag M, Turner GE, Read ND, Seiler S, Bell-Pedersen D, Paietta JV, Plesofsky N, Plamann M, Goodrich-Tanrikulu M, Schulte U, Mannhaupt G, Nargang FE, Radford A, Selitrennikoff C, Galagan JE, Dunlap JC, Loros JJ, Catcheside D, Inoue H, Aramayo R, Polymenis M, Selker EU, Sachs MS, Marzluf GA, Paulsen I, Davis R, Ebbole DJ, Zelter A, Kalkman ER, O'Rourke R, Bowring F, Yeadon J, Ishii C, Suzuki K, Sakai W, and Pratt R. (2004) Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism. Microbiol. Mol. Biol. Rev. 68:1-108. [Free full-text article.]

Sizemore ST and Paietta JV. (2002) Cloning and characterization of scon-3+, a new member of the Neurospora crassa sulfur regulatory system. Eukaryotic Cell 1: 875-883. [Free full-text article.]

Kumar A and Paietta JV. (1998) An additional role for the F-box motif: Gene regulation within the Neurospora crassa sulfur control network. Proc. Natl. Acad. Sci. USA 95, 2417-2422. [Free full-text article.]

Kumar A and Paietta JV. (1995) The sulfur controller-2 negative regulatory gene of Neurospora crassa encodes a protein with beta-transducin repeats. Proc. Natl. Acad. Sci. USA 92, 3343-3347. [Free full-text article.]

Paietta JV. (1995) Analysis of CYS3 regulator function in Neurospora crassa by modification of leucine zipper dimerization specificity. Nucleic Acids Res. 23(6):1044-9. [Free full-text article.]

Paietta JV. (1992) Production of the CYS3 regulator, a bZIP DNA-binding protein, is sufficient to induce sulfur gene expression in Neurospora crassa. Mol Cell Biol. 12(4):1568-77. [Free full-text article.]

Paietta JV. (1990) Molecular cloning and analysis of the scon-2 negative regulatory gene of Neurospora crassa. Mol Cell Biol. 10(10):5207-14. [Free full-text article.]

Paietta JV. (1989) Molecular cloning and regulatory analysis of the arylsulfatase structural gene of Neurospora crassa. Mol Cell Biol. 9(9):3630-7. [Free full-text article.]

Fu, Y-H, Paietta, JV, Mannix, DG, Marzluf, GA (1988) cys-3, the positive-acting sulfur regulatory gene of Neurospora crassa, encodes a protein with a putative leucine zipper DNA-binding element. Mol. Cell. Biol. 9: 1120-1127. [Free full-text article.]

Paietta JV, Akins RA, Lambowitz AM, Marzluf GA. (1987) Molecular cloning and characterization of the cys-3 regulatory gene of Neurospora crassa. Mol Cell Biol. 7(7):2506-11. [Free full-text article.]

Paietta JV, Marzluf GA. (1985) Gene disruption by transformation in Neurospora crassa. Mol Cell Biol. 5(7):1554-9. [Free full-text article.]

Paietta JV, Marzluf GA. (1985) Plasmid recovery from transformants and the isolation of chromosomal DNA segments improving plasmid replication in Neurospora crassa. Curr Genet. 9(5):383-8. [Free full-text article.]

Paietta, J.V. and G.A. Marzluf (1985) Development of shuttle vectors and gene manipulation techniques for Neurospora crassa. In Molecular Genetics of Filamentous Fungi. Alan R. Liss, Inc.: New York, p. 1-13.

Dhawale, S.S., Paietta, J.V. and G.A. Marzluf (1984) A new, rapid and efficient transformation procedure for Neurospora. Current Genetics 8: 77-79. [Free full-text article.]

Paietta, J.V. and G.A. Marzluf (1984) Development of a colony hybridization technique for Neurospora. Fungal Genet. Newsl. 31: 40. [Free full-text article.]

Paietta, J.V. and G.A. Marzluf (1984) Transformation of lithium acetate-treated Neurospora with minipreps of plasmid DNA. Fungal Genet. Newsl. 31:40-41. [Free full-text article.]

Paietta JV, Sargent ML. (1983) Isolation and characterization of light-insensitive mutants of Neurospora crassa. Genetics. 104(1):11-21. [Free full-text article.]

Paietta, J.V. and M.L. Sargent (1983) Modification of blue light responses by riboflavin analogs in Neurospora crassa. Plant Physiol. 72: 764-766. [Free full-text article.]

Paietta, J.V. and M.L. Sargent (1982) Blue light responses in nitrate reductase mutants of Neurospora crassa. Photochem. Photobiol. 35: 853-855.

Paietta JV. (1982) Photooxidation and the evolution of circadian rhythmicity. J Theor Biol. 97(1):77-82. [Abstract.]

Paietta JV, Sargent ML. (1981) Photoreception in Neurospora crassa: correlation of reduced light sensitivity with flavin deficiency. Proc Natl Acad Sci U S A. 78(9):5573-7. [Free full-text article.]

Pao CC, Paietta JV, Gallant JA. (1977) Synthesis of guanosine tetraphosphate (magic spot I) in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 74(1):314-22. [Abstract.]

Paietta JV, McDonald TL, Doermann AH. (1976) Method for the isolation of bacteriphage T4 mutants that produce particles with giant capsids. J Virol. 18(2):785-7. [Free full-text article.]

Clayton DL, Paietta JV. (1972) Selection for circadian eclosion time in Drosophila melanogaster. Science. 178(64):994-5. [Abstract.]


Nucleotide Sequences:

  1. AY135642
    Neurospora crassa rip12b-1 mutant sulfur controller-3 (scon-3) mRNA, complete cds
    gi|27501467|gb|AY135642.1|[27501467]
  2. AF402682
    Neurospora crassa negative regulator sulfur controller-3 (scon-3) gene, complete cds
    gi|22086559|gb|AF402682.1|[22086559]
  3. AY052777
    Neurospora crassa sulfite reductase (cys-4) gene, complete cds
    gi|19068027|gb|AY052777.1|[19068027]
  4. AF404820
    Neurospora crassa methionine synthase (met-8) gene, complete cds
    gi|17385933|gb|AF404820.1|AF404820[17385933]
  5. AF401238
    Neurospora crassa cystathionine gamma-lyase (cys-16) gene, complete cds
    gi|15278112|gb|AF401238.1|AF401238[15278112]
  6. AF401237
    Neurospora crassa cystathionine beta-lyase (met-2) gene, complete cds
    gi|15278107|gb|AF401237.1|AF401237[15278107]
  7. AY050220
    Neurospora crassa homoserine o-acetyltransferase (met-5) gene, complete cds
    gi|15982696|gb|AY050220.1|[15982696]
  8. U89492
    Neurospora crassa arylsulfatase (ars-1) gene, complete cds
    gi|2873362|gb|U89492.1|NCU89492[2873362]
  9. X62506
    N.crassa DNA for autonomously replicating sequence
    gi|2980|emb|X62506.1|NCARSDNA[2980]
  10. U17251
    Neurospora crassa negative regulator sulfur controller-2 (scon-2) gene, complete cds
    gi|806757|gb|U17251.1|NCU17251[806757]
  11. M26008
    Neurospora crassa cys-3 gene, complete cds
    gi|168807|gb|M26008.1|NEUCYS3[168807]