Guoqi Liu, Ph.D., M.B.A.
Research Assistant Professor

Office: 150 Diggs III
Phone: (937) 775-4503
Fax: (937) 775-3730
E-mail: guoqi.liu@wright.edu

Education

Research Interests

Chromosomal instability of short repetitive DNA sequences is responsible for more than 30 human diseases, including more than 20 human neurodegenerative and neuromuscular disorders. Research in my laboratory currently focuses on the DNA replication and DNA damage checkpoint-driven instability of microsatellites at the genomic loci responsible for myotonic dystrophy type1 (DM1) and spinocerebellar ataxia type10 (SCA10). Our goal is to elucidate the mechanisms of chromosomal instability of microsatellites using genetically engineered human cell models in vivo.

Selected Publications

1. Liu, G., Myers, S., Bissler, J., Sinden, R. and Leffak, M. (in preparation ). A triplex-prone DNA sequence from the PKD1 gene forms a polar replication fork barrier in human cells.
2. Liu, G., Bissler, J., Sinden, R. and Leffak, M. (2007). Unstable spinocerebellar ataxia type 10 (ATTCT) (AGAAT) repeats are associated with aberrant replication at the ATX Locus and replication origin-dependent expansion at an ectopic site in human cells. Mol. Cell. Biol. 27: 7828.
3. Gray, S., Liu, G., Altman, A., Small, L., and Fanning, E. (2007). Discrete functional elements required for initiation activity of the Chinese hamster dihydrofolate reductase origin beta at ectopic chromosomal sites. Exp. Cell. Res. 313(1): 109.
4. Ghosh, M., Kemp, M., Liu, G., Ritzi, M., Schepers, A. and Leffak, M. (2006). Differential binding of DNA replication proteins across the human c-myc DNA replicator. Mol. Cell. Biol. 26: 5270.
5. Kemp, M., Ghosh, M., Liu, G. and Leffak, M. (2005). The h istone deacetylase inhibitor trichostatin A alters the pattern of DNA replication origin activity in human cells. Nucleic Acids Res. 33(1): 325.
6. Ghosh, M., Liu, G., Randal, G., Bevington, J. and Leffak, M. (2004). Transcription factor binding and induced transcription alter chromosomal c-myc replicator activity. Mol. and Cell. Biol., 24(23): 10193.
7. Liu, G., Malott, M. and Leffak, M. (2003). Multiple function elements compromise a mammalian replication origin, Mol. and Cell. Biol., 23(5): 1832
8. Yu, C., Xu, J., Tong, Y., Chen, W., Liu, G., Song, H. and Wang, H. (2002). Expression of whole human anti-HAsAg antibody molecule in CHO cells. Bull. Acad. Mil. Med. Sci., 26(3): 176.
9. Tong, Y., Xu, J., Liu, G., Song, H. and Wang, H. (2000). Cloning of human IgG Fc cDNA and expression of whole human anti-HBsAg antibody in CHO cells. Acta Biochem. Biophys. Sinica, 32(4): 392.
10. Liu, G., Chen, X., Xu, J., Song, H., Yu, C., Tong, Y. and Wang, H. (2000). Construction of a CHO cell expression vector carrying amplification gene. J. Cell Mol. Immunol., 16(1): 017.
11. Liu, G. and Wang, H. (2000). Optimized strategies to hyper-express recombinant proteins in CHO cell line, Adv. Biochem. & Biophys., 27(5): 496.
12. Liu, G., Jiang, R. and Zhang, Z. (1999). Integration via Tn10 and expression of cryIVA gene in the chromosome DNA of fluorescent pseudomonads bacterial strains. Chin. J. of Gene., 26(3): 275.
13. Liu, G., Jiang, R. and Zhang, Z. (1999). High-level expression of cryIVB gene of Bacillus thuringiensis var. israelensis in E. coli. Chin. J. Biochem. Mol. Biol., 15: 215.
14. Liu, G., Chen, X., Chen, Z. and Wang, J. (1998). Study on esterase electrophoretic fingerprints of hybrid maize (Zea Ma ys. L) Acta Scient. Natur. Univ. Nankaiensis, 31(4): 6.